Technical Data
I1904-66W
IgG, Lambda (AP)
Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:1000-1:2000. Coat with 1ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Label:
Alkaline Phosphatase
Source: Calf Intestine
Molecular Weight: 140,000D

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse Lambda light chain
Purity:
Prepared by immunoaffinity chromatography followed by solid phase adsorption.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.