Technical Data
I1904-66W
IgG, Lambda (AP)
Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:1000-1:2000. Coat with 1ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Label:
Alkaline Phosphatase
Source: Calf Intestine
Molecular Weight: 140,000D

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4°C Do Not FreezeBlue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse Lambda light chain
Purity:
Prepared by immunoaffinity chromatography followed by solid phase adsorption.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.