Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
ELISA: 1:1000-1:2000. Coat with 1ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Source: Calf Intestine
Molecular Weight: 140,000D
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|1mg||4°C Do Not Freeze||Blue Ice||Mouse||Rabbit|
| ~1mg/ml (UV280)|
|Mouse Lambda light chain|
|Prepared by immunoaffinity chromatography followed by solid phase adsorption.|
|Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|