Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG2b | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | -20°C | Blue Ice | Mouse | Rabbit |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Mouse IgG2b heavy chain |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm. Fluorochrome/Protein Ra |
| Specificity: |
| Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193,1982.
|
|
