Technical Data
IgG (ATTO 590)
A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results. ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 590 shows bright red fluorescence ( abs (max): 594nm, em (max): 624nm, max: 120000).

Suitable for use in Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ATTO 590 conjugates are sensitive to light. Note: We do not recommended adding glycerol as a cryopreservative.
PabIgGAffinity Purified
100ug-20CBlue IceRabbitGoat
Rabbit IgG
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide. Labeled with ATTO 590.
Recognizes the heavy and light chain of rabbit IgG, as well as the light chain of rabbit IgM and IgA. Minimally cross-reacts with human and mouse immunoglobulins. Cross-adsorbed with pooled human sera, purified human paraproteins, pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.