Suitable for use in ELISA. Other applications have not been tested.
ELISA: 5ug/ml as coating antibody
Optimal working dilutions to be determined by researcher.
Coating: I1904-75L1 1gG1
Detection: I1904-76X IgG1 (HRP)
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified mouse IgG1 from BALB/c mice.|
|Purified by affinity chromatography.|
|Supplied as a liquid in PBS, 0.09% sodium azide.|
|Recognizes the gamma 1 heavy chain of mouse immunoglobulin. No crossreactivity with other immunoglobulin classes or subclasses. Does not crossreact with human.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Denis O., Latinne, D., Nisol, F. and Bazin, H. (1993). Resting B cells can act as antigen presenting cells in vivo and induce antibody responses. Int. Immunol. 5: 71-78. 2. Soares, M. et al. (1992) Isotype characterization of rat preformed natural antibodies against guinea pig cells. Transplantation Proceedings 24: 51-452.|