Technical Data
I1904-75M
IgG1
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 3-5ug/ml (coating)
Optimal working dilutions to be determined by researcher.

Recommended Pair:
Suitable for use as the capture or solid phase antibody.
Matched to K0100-01B as the detection or conjugate (label) antibody with purified rat IgG1 as the antigen (Cat# I1904-77G).

Hybridoma:
Sp2/0 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2316Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat IR27 IgG1 myeloma.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes rat IgG1. No crossreactivity is observed with other rat immunoglobulin classes or subclasses.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pelegri, C., et al., Clin. Exp. Immunol. 125: 470-477 (2001). 2. Sato, K., et al., J. Immunol. 166: 4185-4194 (2001).