Suitable for use in Immunohistochemistry and ELISA. Other applications have not been tested.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified Human IgG2.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in PBS, 0.09% sodium azide.|
|Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by haemagglutination assays.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Jefferis, R. et al. (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG sub-classes. Results of an IUIS/WHO collaborative study. Immunology Letters. 10: 223-252.2. Hussain, R. et al. (1986). 2. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of human IgG. J. Immunol. Meth. 93: 89-96. 3. Jefferis, R. et al . (1988). Immunology. 63: 171.|