Technical Data
I1904-80H
IgG2
Description:
Applications:
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:25,000
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase prior to staining.
Optimal dilution determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19A175Affinity Purified
SizeStorageShippingSourceHost
250ug4°C (-20°C Glycerol)Blue IceCanineMouse
Concentration:
~1mg/ml
Immunogen:
Canine IgG
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.2, 0.09% sodium azide.
Specificity:
Recognizes canine IgG2 heavy chain. Does not react with canine IgG1, IgA or IgM. Species Crossreactivity: rabbit
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arce, C. et al. (2002) Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes. Vet. Immunol. Immunopathol. 88:31-41
2. Perez, J. et al. (2002) Comparison of three monoclonal and three polyclonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases. Vet. Dermatol. 13:231-236.