Suitable for use in ELISA. Other applications not tested.
ELISA: 5ug/ml as coating antibody
Optimal dilutions to be determined by the researcher.
Coating: I1904-80I, IgG2a
Detection: I1904-83G1, IgG2a, Heavy Chain (HRP)
Spleen cells from immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified IgG2a from BALB/c mice.|
|Purified by affinity chromatography.|
|Supplied as a liquid in PBS, 0.1% sodium azide.|
|Recognizes mouse gamma 2a heavy chain. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies. PLoS One. 5(4):e10105. 2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J Immunol. 185:7527-36 3. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein. Vaccine. 24: 4102-10. 4. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels. J Allergy Clin Immunol. 122: 633-9.e3.|