Technical Data
I1904-82P
IgG2a, Heavy Chain
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1.25ug/ml
Optimal working dilutions to be determined by researcher.

Avidity for rat IgG2a:
2.1x10e9 L/mole

Matched Pair:
Capture: I1904-82P - IgG2a, Heavy Chain
Detection: K0100-01B - Kappa, Lambda Light Chain
Standard: I1904-82J1 - IgG2a, Kappa, Myeloma, Rat

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse PAI-O myeloma cell line.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-14C: IgG, Fc (HRP) Pab Gt x Mo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2362Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat IgG2a (polyclonal)
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the rat IgG2a heavy chain. Does not crossreact with other rat immunoglobulin classes or subclasses.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazin, H., Beckers, A. & Querinjean, P., Eur. J. Immunol. 4: 44-48 (1974). 2. Kohler, G. & Milstein, C., Nature 256: 495-497 (1975). 3. Schulman, M., Wilde, C.D. & Kohler, G., Nature 276: 269-270 (1978). 4. Bazin, H., Beckers, A., Deckers, C. & Moriame, M., J. Natl. Cancer Institute 51: 1359-1361 (1973). 5. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Platteeu, B. & Urbain, J., J. Immunol. 121: 2077-2082 (1978).