Description:
Applications: Suitable for use in Western Blot, Dot Blot, ELISA, and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution: ELISA: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140Kd)
Storage and Stability: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Type | Isotype | Clone | Grade |
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
| 1mg | | 4°C Do Not Freeze | Blue Ice | Mouse | Rabbit |
|
| Concentration: |
| ~1mg/ml (UV280) |
| Immunogen: |
| Mouse IgG2a heavy chain |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. |
| Specificity: |
| Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Crossreactivity: <1% mouse heavy or light chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
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