Technical Data
IgG2a (Biotin)
Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Other applications not tested.

Recommended Dilution:
ELISA: 1:30,000-1:150,000; I1904-83N was assayed against 1ug of mouse IgG2a in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS (2,2-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.

Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
1mg4C (-20C Glycerol)Blue IceMouseRabbit
Mouse IgG2a heavy chain
Purified by immunoaf nity chromatography.
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
Recognizes mouse IgG2a. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Typically less than 1% crossreactivity was observed against other mouse heavy chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Bayer & Wilchek, Methods in Enzymology 184: 138-140 (1990).