Technical Data
IgG2a (HRP)
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:5000-1:20,000
Western Blot: 1:1000 - 1:5000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
PabIgGAffinity Purified
1mg-20°CBlue IceMouseRabbit
~1mg/ml (after reconstitution)
Mouse IgG2a (heavy chain).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Recognizes mouse IgG2a. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, mouse IgG and mouse serum. Specificity was confirmed by ELISA 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Farr & Nakane, J. Immunol. Methods 47: 129-144 (1981).