Technical Data
I1904-83S
IgG2a (TRITC)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label:
Tetramethylrhodamine isothiocyanante (TRITC) (MW 444D)

Absorption Wavelength:
550 nm

Emission Wavelength:
570 nm

Fluorochrome/Protein Ratio:
2.1 moles TRITC per mole of Rabbit IgG

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. TRITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse IgG2a chain
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with tetramethylrhodamine isothiocyanante (TRITC).
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, mouse IgG and mouse serum. < 1% Crossreactivity against other mouse or human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.