Technical Data
I1904-83T
IgG2a, X-Adsorbed (FITC)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:1000 -1:5000
FLISA: 1:10,000- 1:50,000
Optimal dilutions to be determined by researcher.

Label: Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 3.6 moles FITC per mole of rabbit IgG

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse IgG2a heavy chain
Purity:
Prepared by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes mouse IgG2a heavy chain. Crossreactivity against other mouse or human heavy or light chain isotypes is <1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.