Technical Data
I1904-84C
IgG2b (AP)
Description:
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Application(s): suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Recommended Dilution(s): This product has been assayed against 1ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Storage Conditions: Store vial at 4 C before opening. DO NOT FREEZE. This product is stable at 4 C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceMouseRabbit
Concentration:
1.0 mg/ml (UV280)
Immunogen:
Mouse IgG2b heavy chain
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form
Apconj Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.