Technical Data
IgG2b (Biotin)
Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested.

Recommended Dilution:
ELISA: 1:20,000 to 1:100,000 using ABTS as substrate for 30 min at RT.
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
1mg-20CBlue IceMouseRabbit
~1mg/ml (UV280)
Mouse IgG2b heavy chain
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile, ddH2O.
Recognizes mouse IgG2b. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other mouse heavy chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.