Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
ELISA: 1:20,000 to 1:100,000
Western Blot: 1:2000-1:10,000
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C upon receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| ~1mg/ml (after reconstitution)|
|Mouse IgG2b heavy chain|
|Purified by immunoaffinity chromatography.|
|Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.|
|Recognizes mouse IgG2b. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other mouse heavy chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|