Technical Data
I1904-84E
IgG2b (FITC)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:500-1:2500
Optimal working dilutions to be determined by researcher.

Label: Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Rabbit IgG

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse IgG2b.
Purity:
Prepared by immunoaffinity chromatography.
Form
Supplied as a lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in sterile 40-50% glycerol, dH2O.
Specificity:
Recognizes mouse IgG2b, g2b chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.