Technical Data
I1904-84H
IgG2b
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 5ug/ml (as a coating antibody)
Optimal working dilutions to be determined by researcher.

Avidity for rat IgG2b: 4.7 x 10e9 M-1.

Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2401Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat IgG2b myeloma protein (IR863).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sato, K., et al., Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat transplant. J. Immunol. 166: 4185-4194 (2001).