Description:
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Applications:
Flow Cytometry
ELISA: 5ug/ml as coating antibody
Optimal working dilutions to be determined by researcher.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 3H2412 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ug | | -20°C | Blue Ice | Rat | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| IgG2c (Rat strain LOU/c). |
| Purity: |
| Purified by affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Specificity: |
| Rat gamma 2c heavy chain (determined by immunodot). The specificity of monoclonal antibodies against IgG subclasses is determined in a range of optimal concentrations. Increasing the concentration of the first or second antibody can induce cross-reactions with other subclasses. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Manouvriez, P., Lefebvre, M., Genart, C., Cormont, F. & Bazin, H. (1986). Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by ELISA with mouse monoclonal antibodies. Immunobiology 173: 307-308.
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