Technical Data
I1904-85A
IgG3 (TRITC)
Description:
Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)

Absorption Wavelength: 550 nm

Emission Wavelength: 570 nm

Fluorochrome/Protein Ratio: 2.5 moles TRITC per mole of Rabbit IgG


Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher.

Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
IgG3 g3 chain
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Labeled with TRITC.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgG3. Speci city was con rmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.