Technical Data
I1904-85G
IgG3 (Biotin)
Description:
Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000 to 1:32,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse IgG3 heavy chain.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycero
Specificity:
Recognizes mouse IgG3. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other Mouse heavy chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Huntley, J. F. et al., (2008) Infection and Immunity, 76:3664-3671. 1. Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.