Technical Data
IgG3 (Biotin)
Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000 to 1:32,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
1mg4C (-20C Glycerol)Blue IceMouseRabbit
~1mg/ml (UV280)
Mouse IgG3 heavy chain.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycero
Recognizes mouse IgG3. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other Mouse heavy chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Huntley, J. F. et al., (2008) Infection and Immunity, 76:3664-3671. 1. Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.