Description:
Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested.
Recommended Dilution:
ELISA: 1:8000 to 1:32,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | 4°C (-20°C Glycerol) | Blue Ice | Mouse | Rabbit |
|
| Concentration: |
| ~1mg/ml (UV280) |
| Immunogen: |
| Mouse IgG3 heavy chain. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycero |
| Specificity: |
| Recognizes mouse IgG3. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other Mouse heavy chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
US Biological application reference: Huntley, J. F. et al., (2008) Infection and Immunity, 76:3664-3671. 1. Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.
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