Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested.
ELISA: 1:8000 to 1:32,000
Western Blot: 1:1000-1:5000
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|1mg||4°C (-20°C Glycerol)||Blue Ice||Mouse||Rabbit|
| ~1mg/ml (UV280)|
|Mouse IgG3 heavy chain.|
|Purified by immunoaffinity chromatography.|
|Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycero|
|Recognizes mouse IgG3. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other Mouse heavy chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
US Biological application reference: Huntley, J. F. et al., (2008) Infection and Immunity, 76:3664-3671. 1. Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.|