Suitable for Immunoblotting (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested.
ELISA: 1:2000-1:4000 Use pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.
Alkaline Phosphatase (Calf Intestine) (MW 140kD)
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|1mg||4°C Do Not Freeze||Blue Ice||Mouse||Rabbit|
| ~1mg/ml (UV280)|
|Mouse IgG3 heavy chain|
|Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.|
|Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphotase (AP).|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Avarameas and Ternyrock, Immunochemistry 32; 1175-1971.|