Suitable for use in Western Blot and Immunohistochemistry. Not suitable for use with paraffin sections. Other applications not tested.
Immunohistochemistry: Frozen Not suitable for use with paraffin sections.
Optimal dilutions to be determined by the researcher.
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified human IgG4|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in Borate buffered saline, 0.09% sodium azide.|
|Recognizes human IgG4, at an epitope in the pFc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: Chimpanzee|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Jefferis, R. et al. (1985). Immunology Letters. 10:223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34:271-8. 3. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders. J Clin Pathol. Jan 12. [Epub ahead of print]. 4. Black, C.M. et al. (1991) Immunology. 72:94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9:29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46:195-8.|