Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Mouse IgM mu heavy chain|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.|
|Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgM and mouse serum. No reactivity was observed against other mouse heavy or light chain proteins.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Bayer & Wilchek, Methods in Enzymology, 184: 138-160 (1990).|