Technical Data
I1906-02B
IgY (HRP)
Description:
The antibody can be used during various purification steps of IgY. The yolk of eggs laid by immunized chickens has been recognized as an excellent source of polyclonal antibodies (pAb). Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. Because a single egg contains as much antibody as an average bleed from a rabbit, this simple, non-invasive approach presents an appealing alternative to conventional pAb production methods. Purification of chicken egg yolk immunoglobulin Y (IgY), the 150kD IgG homolog, does not require animal bleeding. In addition, the eggs from immunized chickens provide a continual, daily source of pAb, and this convenient approach offers greater compatibility with animal protection regulations. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens. Highly conserved mammalian proteins sometimes fail to illicit a humoral response in animals, such as rabbits, that are traditionally used for generating pAb. Non-specific binding and need for cross-species immunoabsorptions is eliminated since chicken IgY does not cross-react with mammalian IgG and does not bind bacterial or mammalian Fc receptors.
There are well defined structural differences of IgY-type immunoglobulins and the IgG of mammals. That includes the molar mass of the heavy chains of the immunoglobulins. The IgY-type immunoglobulins are much less flexible than IgG. Also, the structures of the Fc part of the immunoglobulin isotypes IgY and IgG are different.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot (Non-reduced): 1:10
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.áLabeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG16A557Purified
SizeStorageShippingSourceHost
50ug-20░CBlue IceChickenMouse
Concentration:
~0.1mg/ml
Immunogen:
Chicken IgY.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% 2-chloroacetamide. Labeled with HRP.
Specificity:
Recognizes chicken IgY. Species Crossreactivity: duck.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.