Technical Data
I3000-27B
IKK beta (IkappaB Kinase beta, IKKb, IKK2)
Description:
NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB. In turn IkB is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b which phosphorylate I B leading to its degradation and the resulting translocation of NFkB to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-a endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:5000
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
I3000-27C

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to a region near the C-terminus of the human IkBb protein (KLH)
Purity:
Serum
Form
Supplied as a liquid in 0.1% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human IkBb protein at 87kD. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.