Technical Data
I5000-05P
Interleukin 1 Receptor AcP, Control Peptide (IL1RacP, IL-1 Receptor Accessory Protein)
50ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Peptide corresponding to aa525-540 of human IL-1RAcP, which is identical to those of mouse and rat origins
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Applications:
Suitable for use as blocking peptide in Western Blot with I5000-05.

Recommended Dilution:
Blocking: Incubation of I5000-05P with equal volume of I5000-05 for 30 min. at 37C typically blocks antibody activity in Western Blot completely.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Synthetic peptide
Concentration: ~0.2mg/ml
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Greenfeder, S.A., Nunes, P., Kwee, L., Labow, M., Chizzonite, R.A., Ju, G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem (1995) 270: 13757-13765. 2. Huang, J., Gao, X., Li, S., Cao, Z. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein. Proc Natl Acad Sci U S A (1997) 94: 12829-12832. 3. Wesche, H., Korherr, C., Kracht, M., Falk, W., Resch, K., Martin, M.U. The interleukin-1 receptor accessory protein (IL-1RAcP) is essential for IL-1-induced activation of interleukin-1 receptor-associated kinase (IRAK) and stress-activated protein kinases (SAP kinases). J Biol Chem (1997) 272: 7727-7731. 4. Hofmeister, R., Wiegmann, K., Korherr, C., Bernardo, K., Kronke, M., Falk, W. Activation of acid sphingomyelinase by interleukin-1 (IL-1) requires the IL-1 receptor accessory protein. J Biol Chem (1997) 272: 27730-27736.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.