Technical Data
I7000
Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole)
100g
500g
1Kg
Biochemicals Storage: 4CShipping: RT
Imidazole can be used for the elution of histidine containing proteins from divalent cation resins.

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25C. It is also a chelator for the binding of various divalent cations.

The logs of the binding constants for various divalent cations are below:
Divalent Cation Log binding constant
Ca2+ 0.1
Mn2+ 1.6
Fe2+ 3.3
Co2+ 2.4
Ni2+ 2.9
Cu2+ 4.2
Zn2+ 2.0

Solubility (10%):
Colorless, clear, complete
Imidazole is soluble in dH2O (~500mg/ml).

Boiling Point: ~256C

Melting Point: 88-92C

Storage and Stability:
Powder may be stored at 4C. Stable for 12 months at -20C after receipt. Reconstitute with ddH2O. Aliquot and store at 4C. Reconstituted product is stable for 6 months at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.

CAS Number:
288-32-4

Molecular Formula:
C3H4N2

Molecular Weight:
68.08
Purity: ~99%
Form: Colorless to pale yellow crystalline flakes

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), 324-325. 2. The Merck Index, 13th ed., Entry# 4935. 3. Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), 433, 409, 404-405. 4. Fernandez-Patron, C., et al., Biotechniques 12(4): 564-573 (1992).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.