Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole)
|Biochemicals||Storage: 4°CShipping: RT|
Imidazole can be used for the elution of histidine containing proteins from divalent cation resins.
Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is also a chelator for the binding of various divalent cations.
The logs of the binding constants for various divalent cations are below:
Divalent Cation Log binding constant
Colorless, clear, complete
Imidazole is soluble in dH2O (~500mg/ml).
Boiling Point: ~256°C
Melting Point: 88-92°C
Storage and Stability:
Powder may be stored at 4°C. Stable for 12 months at -20°C. Reconstitute with ddH2O Aliquot and store at 4°C. Reconstituted product is stable for 12 months at -4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.
Form: Colorless to pale yellow crystalline flakes
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), 324-325. 2. The Merck Index, 13th ed., Entry# 4935. 3. Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), 433, 409, 404-405. 4. Fernandez-Patron, C., et al., Biotechniques 12(4): 564-573 (1992).