Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole)
|Biochemicals||Storage: 4°CShipping: RT|
Imidazole can be used for the elution of histidine containing proteins from divalent cation resins.
Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is also a chelator for the binding of various divalent cations.
The logs of the binding constants for various divalent cations are below:
Divalent Cation Log binding constant
Colorless, clear, complete
Imidazole is soluble in dH2O (~500mg/ml).
Boiling Point: ~256°C
Melting Point: 88-92°C
Storage and Stability:
Powder may be stored at 4°C. Stable for 12 months at -20°C. Reconstitute with ddH2O Aliquot and store at 4°C. Reconstituted product is stable for 12 months at -4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.
Form: Colorless to pale yellow crystalline flakes
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.