Technical Data
I7000
Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole)
100g
500g
1Kg
500g
1Kg
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Technical Data |
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I7000 |
Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole) |
100g 500g 1Kg |
| Biochemicals | Storage: 4°CShipping: RT |
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Imidazole can be used for the elution of histidine containing proteins from divalent cation resins. Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is also a chelator for the binding of various divalent cations. The logs of the binding constants for various divalent cations are below: Divalent Cation Log binding constant Ca2+ 0.1 Mn2+ 1.6 Fe2+ 3.3 Co2+ 2.4 Ni2+ 2.9 Cu2+ 4.2 Zn2+ 2.0 Solubility (10%): Colorless, clear, complete Imidazole is soluble in dH2O (~500mg/ml). Boiling Point: ~256°C Melting Point: 88-92°C Storage and Stability: Powder may be stored at 4°C. Stable for 12 months at -20°C. Reconstitute with ddH2O Aliquot and store at 4°C. Reconstituted product is stable for 12 months at -4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light. CAS Number: 288-32-4 Molecular Formula: C3H4N2 Molecular Weight: 68.08 |
Purity: ~99% Form: Colorless to pale yellow crystalline flakes Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), 324-325. 2. The Merck Index, 13th ed., Entry# 4935. 3. Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), 433, 409, 404-405. 4. Fernandez-Patron, C., et al., Biotechniques 12(4): 564-573 (1992).
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