Technical Data
Immunoprecipitation (IP) Assay Kit, Reversible, BioAssay™
Kits and Assays Storage: 4°CShipping: Blue Ice
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP’s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in this kit is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. This IP kit is not suitable to immunoprecipitate proteins expressing 6X Histidine epitope tagged fusion proteins (i.e., His-tagged fusion proteins) due to non-specific interactions with the resin.

Kit Components:
I7505-15A: Antibody Capture Affinity Ligand, (ACAL) 1x1vial
I7505-15B: Wash Buffer (10X), 1x15ml.
I7505-15C: Non-denaturing Elution Buffer (4X), 1x10ml
I7505-15D: Denaturing Elution Buffer (1X), 1x4ml
I7505-15E: Spin Column, 50x1 column containing 0.5 ml (20% w/v) of IP capture resin in suspension.
I7505-15F: Capture Tube, 100x1 reservoir tubes..

Storage and Stability:
Store all components at 4°C, except I7505-15F: Capture Tube which can be stored at RT. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Morgan, K. (2003) Protein Labeling and Immunoprecipitation. Current Protocols in Cell Biology, (J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz, and K.M. Yamada, eds.) Volume 1, Chapter 7, pp. 7.2.1-7.2.14. John Wiley & Sons. 2. Harlow, E. and Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 3. Springer, T.A. (1996) Immunoprecipitation. In Current Protocols in Immunology (J.E. Coligan, A.M. Kruisbeck, D.H. Margulies, E.M. Shevach, and W. Strober, eds.) pp.8.3.1-8.3.11. John Wiley & Sons, New York. 4. Irving, R.A., Hudson, P.J., and Goding, J.W. (1996) Construction, screening and expression of recombinant antibodies. In Monoclonal Antibodies: Principals and Practice (J.W. Goding, ed.) academic Press, London. 5. Nisonoff, A. (1984) Introduction to Molecular Immunology. Sinauer Associates, Sunderland Mass. 6. Rapley, R. (1995) The biotechnology and applications of antibody engineering. Mol. Biotech. 3: 139-154. 7. Gersten, D.M. and Marchalonis, J.J. (1978) A rapid, novel method for the solid-phase derivatization of IgG antibodies for immuno-affinity chromatography. J. Immunological Methods 24: 305-309.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.