Technical Data
I7649-52
Influenza A H5N1 (Avian) Nonstructural Protein 1 (NS1)
Description:
The NS1 protein of influenza A virus (NS1A protein) is a multifunctional protein that counters cellular antiviral activities and is a virulence factor. The influenza protein NS1 is a 26,000 Dalton protein. Human messenger RNA carries a poly-A tail at the 5' end. NS1 forms a dimer that inhibits the export of poly-A containing mRNA molecules from the nucleus. This gives preference to viral RNA which is transported to the ribosome to be translated. NS1 might also inhibit splicing of pre-mRNA by binding to a stem-bulge region in U6 small nuclear RNA (snRNA). In addition, NS1 is probably able to suppress the interferon response in the virus-infected cell leading to unimpaired virus production. NS1 also binds dsRNA. The NS1 protein of the highly pathogenic avian H5N1 viruses circulating in poultry and waterfowl in Southeast Asia is currently believed to be responsible for the enhanced virulence of the strain. H5N1 NS1 is characterized by a single amino acid change at position 92. By changing the amino acid from glutamic acid to aspartic acid, researchers were able to annul the effect of the H5N1 NS1. This single amino acid change in the NS1 gene greatly increased the pathogenicity of the H5N1 influenza virus.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa in the carboxy terminus of the NS1 protein (Genbank No. ABC72653).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes Nonstructural protein 1 (NS1) from an H5N1 strain of avian influenza A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thompson, W.W., et al., (2003), "Mortality associated with influenza and respiratory syncytial virus in the United States", JAMA, 289: 179-186, 2. Alexander, D.J., (2000), "A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals", Vet. Microbiol., 74, 3-13, 3. Krug, R.M., et al., (2003), "Intracellular warfare between human influenza viruses and human cells: the role of the viral NS1 protein", Virology, 309: 181-189, 4. Min, J-Y. and Krug, R.M., (2006), "The primary function of RNA binding by the influenza A virus NS1 protein is infected cells: inhibiting the 2'-5' oligo (A) synthase/RNase L pathway", Proc. Natl. Acad. Sci. USA, 103: 7100-7105, 5. Li, S., et al., (2006), "Binding of the influenza A NS1 protein to PKR mediates the inhibition of its activation by either PACT or double-stranded RNA", Virology, 349: 13-21.