Technical Data
I7649-54
Influenza A H5N1 (Avian) Nonstructural Protein 2 (NS2)
Description:
Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species jumping ability.

H5 stands for the fifth of several known types of the viral protein hemagglutinin. This protein binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa near the middle of the NS2 protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes Nonstructural protein 2 (NS2) from an H5N1 strain of avian influenza A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thompson, W.W., et al., (2003), "Mortality associated with influenza and respiratory syncytial virus in the United States", JAMA, 289: 179-186, 2. Alexander, D.J., (2000), "A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals", Vet. Microbiol., 74, 3-13, 3. Neumann, G., et al., (2000), "Influenza A virus NS2 protein mediates vRNP nuclear export through NES-independent interaction with hCRM1", EMBO J., 19: 6751-6758, 4. Garcia-Robles, I., et al., (2005), "Interaction of influenza virus proteins with nucleosomes", Virology, 332: 329-336.