Technical Data
I7650-04P
Influenza A
Description:
Influenza viruses are common and widely spread infectious agents. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system.

Applications:
Suitable for use in ELISA (1). Not suitable for use in Western Blot (1). Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5D8Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Influenza A.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity:
Specific for influenza A virus nucleoprotein (1). Epitope differs from that of clone 6F132. A pool of clones 6F131 and 6F132 is suitable as both capture and detection antibody in sandwich ELISA (1). Reacts with influenza A infected, acetone-fixed Vero cells when diluted 1:1000. Specific binding can be visualized by immunofluorescence using a polyclonal anti-mouse Ig antibody labeled with, for example, fluorescein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Glikmann G, Mordhorst CH, Koch C (1994) Monoclonal antibodies for the direct detection of influenza-A virus by ELISA in clinical specimens from patients with infections. Clinical and diagnostic virology 3:361-369.