Influenza viruses are common and widely spread infectious agents. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system.
Suitable for use in ELISA (1). Not suitable for use in Western Blot (1). Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Purified by Protein A/G affinity chromatography from culture supernatant.|
|Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.|
|Specific for influenza A virus nucleoprotein (1). Epitope differs from that of clone 6F132.A pool of clones 6F131 and 6F132 is suitable as both capture and detection antibody in sandwich ELISA (1). Reacts with influenza A infected, acetone-fixed Vero cells when diluted 1:1000. Specific binding can be visualized by immunofluorescence using a polyclonal anti-mouse Ig antibody labeled with, for example, fluorescein.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Glikmann G, Mordhorst CH, Koch C (1994) Monoclonal antibodies for the direct detection of influenza-A virus by ELISA in clinical specimens from patients with infections. Clinical and diagnostic virology 3:361-369.|