Technical Data
I7650-04S
Influenza A Neuraminidase N1 (Swine-origin California/14/2009, Georgia/20/2006, H1N1)
Description:
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). Novel influenza virus strains emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found (2). These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3). There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low (4). Although it has been known that cleavage site and glycosylation patterns of the HA protein play important roles in determining the pathogenicity of H5 avian influenza viruses, it has only recently been shown that an additional glycosylation site within the globular head of the NA protein also contributes to the high virulence of the H5N1 virus (5).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the neuraminidase N1 protein of Swine origin Influenza A/California/14/2009 (H1N1) and neuraminidase N1 protein of seasonal Influenza A/Georgia/20/2006 (H1N1) (ACA33620:ACQ76308).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes the neuraminidase N1 peptide from the Swine-origin Influenza A/California/14/2009 (H1N1) and seasonal Influenza A/Georgia/20/2006 (H1N1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thompson, W.W., et al., (2003), “Mortality associated with influenza and respiratory syncytial virus in the United States,” JAMA, 289:179-186. 2. Dawood, F.S., et al., (2009), “Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Emergence of a novel swine-origin influenza A (H1N1) virus in humans,” N. Engl. J. Med., 360:2605-2615. 3. Butler D., (2009), “Swine flu goes global,” Nature, 458:1082-1083. 4. Morens, D.M., et al., (2009), “The Persistent Legacy of the 1918 Influenza Virus,” N. Engl. J. Med., June 29. 5. Garten R.J., et al., (2009), “Antigenic and Genetic Characteristics of Swine-Origin 2009 A (H1N1) Influenza Viruses Circulating in Humans,” Science, May 22.