Technical Data
I7650-81J
Influenza A Nucleoprotein (NP)
Description:
The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from BALB/c mice.

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG38J92Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purified Influenza A/New Caledonia/20/99 (H1N1)
Purity:
Purified by Protein A chromatography. >90% pure (SDS-PAGE).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes Nucleoprotein (NP) of Influenza A. Does not cross react with nucleoprotein of Influenza B virus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.