Technical Data
ING1 (Inhibitor of Growth 1)
ING1(inhibitor of growth-1) is a member of the ING family of tumor suppressive and apoptosis-promoting epigenetic regulators of chromatin structure. They interact with and alter the ratio of pro- and anti-apoptotic Bcl-2 family members. The p53 tumor suppressor and its downstream targets can potentiate ING1-induced apoptosis. Human isoforms p33ING1, p24ING1c, p33ING1b and p47ING1a vary in N-terminal sequence, resulting in 279, 210, 235 and 422aa proteins, respectively. The sequence used as an immunogen is common to all isoforms, and shares 83% and 81% aa identity with mouse and rat ING1, respectively.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG111D48Affinity Purified
100ug-20CBlue IceHumanMouse
Not determined
Recombinant corresponding to aa70-184 from human ING1 expressed in E. coli (Q9UK53).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Recognizes human and mouse ING1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.