Technical Data
I7652-53D
ING1 (Inhibitor of Growth Protein 1)
Description:
ING1 (inhibitor of growth-1) is a member of the ING family of tumor suppressive and apoptosis-promoting epigenetic regulators of chromatin structure. They interact with and alter the ratio of pro- and anti-apoptotic Bcl-2 family members. The p53 tumor suppressor and its downstream targets can potentiate ING1-induced apoptosis. Human isoforms p33ING1, p24ING1c, p33ING1b and p47ING1a vary in N-terminal sequence, resulting in 279, 210, 235-422aa proteins, respectively. The sequence used as an immunogen is common to all isoforms, and shares 83-81aa identity with mouse and rat ING1, respectively.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG111n85Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa70-184 from human ING1 expressed in E. coli (Q9UK53).
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human ING1. No cross-reactivity with recombinant human ING2, 3, 4, or 5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.