Technical Data
Media
Inositol Assay Medium
I7655-05
Cell Culture Grade
Storage 4C    Shipping RT
Formulation:
DL-Alanine0.4g
L-Arginine0.48g
L-Asparagine 0.8g
DL-Aspartic Acid0.2g
Biotin
Calcium Chloride0.25g
Citric Acid2.0g
L-Cystine0.1g
Ferric Chloride50.0mg
Dextrose100.0g
L-Glutamic Acid0.6g
Glycine0.2g
L-Histidine124.0mg
L-Isoleucine0.5g
L-Leucine0.5g
L-Lysine0.5g
Magnesium Sulfate0.25g
Manganese Sulfate50.0mg
L-Methionine0.2g
Calcium Pantothenate5.0mg
DL-Phenylalanine0.2g
Potassium Chloride0.85g
Potassium Citrate10.0g
Monopotassium Phosphate1.1g
L-Proline0.2g
Pyridoxine Hydrochloride1.0mg
DL-Serine0.1g
DL-Threonine0.4g
Thiamine Hydrochloride500.0ug
DL-Tryptophan80.0mg
L-Tyrosine0.2g
L-Valine0.5g
Inositol Assay Medium is used for determining inositol concentration by the microbiological assay technique. A modification of the formula described by Atkin et al.,1 is used in the microbiological assay of inositol using Saccharomyces cerevisiae ATCCTM 9080 (Saccharomyces uvarum) as the test organism.


Directions per Liter: Dissolve 12.2g in 100ml of ddH2O stirring with heat. Boil for 1 minute until completely solubilized. Dispense 5ml amounts into tubes. Add standard or test samples. Adjust tube volume to 10ml. Autoclave at 121C for 5 minutes. See Difco Manual for more directions.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White to off-white, homogeneous, free flowing powder

Solubility: Colorless to slightly yellow, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.