Technical Data
I7656-01B
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It is produced by B cells of the pancreas. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage,formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100. Does not require protein digestio or antigen retrieval using heat treatment.
ELISA: 5ng/ml as a detection reagent.
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo

Recommended IHC Positive Control Tissue:
Pancreas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2464Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Insulin conjugated to BSA
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide. No glycerol added.
Specificity:
Recognizes human insulin. Species Crossreactivity: Porcine, rabbit, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.