Technical Data
I7656-05
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in ELISA (two-site enzyme immunoassay). Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the capture or solid phase antibody.
Matched to I7656-04 as the detection or conjugate (label) antibody.

Hybridoma:
X63-Ag8-653 myeloma cells with spleen cells from BALB/c mice.

Affinity Constant:
8.0x10e8 M e-1

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2B60Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Purified human insulin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human insulin. Species Crossreactivity: human proinsulin, porcine insulin and bovine insulin (30%). No crossreaction with free C-peptide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.