Technical Data
I7656-06
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the capture or solid phase antibody.
Matched to I7656-07 as the detection or conjugate (label) antibody.

Affinity Constant:
7.5 x 10e9 M-1

Hybridoma:
Hybridization of X63-Ag8653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommend adding glycerol as a cryopreservative.
TypeIsotypeCloneGrade
MabIgG1,k1.B.105Affinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Purified human insulin.
Purity:
Purified by Protein A affinity chromatography (> 90%, SDS-PAGE).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. No glycerol added.
Specificity:
Recognizes human Insulin. Does not crossreact wtih free C-peptide. Species Crossreactivity: Human proinsulin, bovine insulin (30%) and porcine insulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.