Technical Data
I7656-07
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the detection or conjugate (label) antibody.
Matched to I7656-06 as the capture or solid phase antibody.

Hydridoma:
X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.

Affinity Constant:
1.5 x 10e8 M-1

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommend adding glycerol as a cryopreservative.
TypeIsotypeCloneGrade
MabIgG1,k1.B.106Affinity Purified
SizeStorageShippingSourceHost
1mg4CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Purified human insulin
Purity:
Purified by Protein A affinity chromotography (> 90% by electrophoresis).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Glycerol free.
Specificity:
Recognizes human insulin. Does not crossreact with free C-peptide. Species Crossreactivity: Human proinsulin, bovine (30%) and porcine insulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.