Technical Data
I7656-10
Insulin (Biotin)
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in detection of insulin in two-site enzyme immunoassay. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.

Affinity Constant:
8.0x10e8L/mole

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2B60Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human insulin
Purity:
Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
Form
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1% sodium azide. Labeled with Biotin.
Specificity:
Recognizes human insulin. Crossreacts with human proinsulin, bovine insulin (30%) and porcine insulin. No crossreaction with free C-peptide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.