Technical Data
I7656-17
Insulin
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Technical Data |
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I7656-17 |
Insulin |
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Description: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (FITC): 1:400-1:800 with overnight incubation at 4°C Immunohistochemistry: Frozen and formalin-fixed paraffin sections Optimal dilutions to be determined by the researcher. Positive Control: Formalin-fixed paraffin sections of porcine pancreas. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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