Technical Data
I7656-17
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence (FITC): 1:400-1:800 with overnight incubation at 4C
Immunohistochemistry: Frozen and formalin-fixed paraffin sections
Optimal dilutions to be determined by the researcher.

Positive Control:
Formalin-fixed paraffin sections of porcine pancreas.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IcePorcineGuinea pig
Concentration:
Not determined
Immunogen:
Porcine insulin
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH2O. Dilute further in sterile 0.1MPBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes porcine insulin. Does not crossreact with polypeptide hormones unrelated to insulin. Species Crossreactivity: human, rat, ferret.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Ahren B et al: Cysteamine and the endocrine pancreas: immunocytochemical, immunochemical and functional aspects. Cell Tissue Res 256, 159-166 (1989)
Luts A et al: Peptide-containing nerve fibers in the respiratory tract of the ferret. Cell Tissue Res 258, 259-267 (1989)