Technical Data
I7656-18
Insulin
Description:
Insulin is produced by the B-cells of the pancreatic islets. Antigen localization: cytoplasm, extracellular.

Applications:
Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin sections, Proteolytic treatment not required; optimal results have been obtained by using the autoclave or microwave retrieval method (with citrate buffer))

Recommended Dilution:
Immunohistochemistry: 1:50 -1:100; Based on the indirect method, with a normal ABC method the dilution can be up to 5X higher when using the microwave retrieval method, but the optimal dilution should be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
250ul-20CBlue IcePorcineRabbit
Concentration:
Not determined
Immunogen:
Porcine insulin.
Purity:
Serum
Form
Supplied as a liquid.
Specificity:
Can be used for the diagnosis of pancreatic B-cell tumors. Recommended for Pancreas. Species Crossreactivity: Human, porcine, rat, ferret.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Luts A et al: Peptide-containing nerve fibers in the respiratory tract of the ferret. Cell Tissue Res 258, 259-267 (1989)