		Technical Data
I7656-18	Insulin

Description:
Insulin is produced by the B-cells of the pancreatic islets. Antigen localization: cytoplasm, extracellular.

Applications:
Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin sections, Proteolytic treatment not required; optimal results have been obtained by using the autoclave or microwave retrieval method (with citrate buffer))

Recommended Dilution:
Immunohistochemistry: 1:50 -1:100; Based on the indirect method, with a normal ABC method the dilution can be up to 5X higher when using the microwave retrieval method, but the optimal dilution should be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Porcine	Rabbit

Concentration:

Not determined

Immunogen:

Porcine insulin.

Purity:

Serum

Form

Supplied as a liquid.

Specificity:

Can be used for the diagnosis of pancreatic B-cell tumors. Recommended for Pancreas. Species Crossreactivity: Human, porcine, rat, ferret.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Luts A et al: Peptide-containing nerve fibers in the respiratory tract of the ferret. Cell Tissue Res 258, 259-267 (1989)