Technical Data
I7660-16AX
Insulin (BSA & Azide Free)
Description:
Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as b-cell and tumor (insulinoma) marker.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 minutes at RT. No special pretreatment is required.
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3F209Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified Insulin protein. Cellular Localization: Cytoplasmic. MW of Antigen: 6kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. No glycerol added. Also available with BSA and azide. See I7660-16A.
Specificity:
Recognizes human Insulin. Excellent for identifying the beta-cells of islets of Langerhans in routine formalin-fixed, paraffin-embedded pancreatic tissues. Species Crossreactivity: porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.