Technical Data
I7660-16X
Insulin (BSA & Azide Free)
Description:
Insulin is a 51-amino acid polypeptide (~6kD) composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as b-cell of islets of Langerhans and tumor (insulinoma) marker.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 30 min RT. No special pretreatment required.
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F213Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Porcine insulin. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. No glycerol added. Also available with BSA and azide. See I7660-16
Specificity:
Recognizes human Insulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.