Technical Data
I7660-25A
Insulin
Description:
Glucose homeostasis is regulated by hormones. Elevations in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined by a disulfide bond to form insulin, and the central part of the precursor molecule is cleaved and released as C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).

Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:100
Immunofluorescence (Frozen): 1:100
Immunohistochemistry (Paraffin): 1:100. Antigen retrieval: heat in citrate buffer. Dilute antibody in TBST/5% normal goat serum.
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide cooresponding to the sequence of human insulin.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human insulin protein. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Straub, S.G. & Sharp, G.W., Diabetes Metab. Res. Rev. 18: 451–463 (2002). 2. Concannon, P., et al., Nat. Genet. 19: 292–296 (1998).