Technical Data
I7660-25C
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:500 (PAP or ABC)
Immunofluorescence: 1:200
Optimal dilutions to be determined by the researcher.

Amino Acid Sequence:
Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn-Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human Insulin.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Specificity:
Recognizes human Insulin. Species Crossreactivity: rat and canine. Does not react with sheep. Does not react with C-Peptide, Somatostatin, Glucagon, Human Amylin, Human Pancreatic Polypeptide or Human IGF-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.