Technical Data
I7660-30A1
Insulin, Proinsulin (Biotin)
Description:
Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Affinity Constant:
6.3x10e-8M

Recommended Pair:
Capture: I7660-30B
Detection: I7660-30A

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: We do not recommended adding glycerol as a cryopreservative.
TypeIsotypeCloneGrade
MabIgG1,k1.B.115Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human insulin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 20mM Tris-HCl, 150mM sodium chloride,, pH 7.5, 0.1% sodium azide. Labeled with Biotin. Also available unconjugated. See I7660-30A.
Specificity:
Recognizes human Insulin, Proinsulin. Species Crossreactivity: bovine, porcine insulin and proinsulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.