Technical Data
I7661-10J
Insulin-Like Growth Factor I, Recombinant, Mouse (IGF1)
10ug
50ug
Molecular Biology Storage: -70CShipping: Blue Ice
Insulin-Like Growth Factor I, Recombinant, Murine (IGF1) is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.

Biological Activity: IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10U/mg.

AA Sequence:
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
7.6kD
Source: Recombinant, murine, E. coli
Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Disturbed cross talk between insulin-like growth factor I and AMP-activated protein kinase as a possible cause of vascular dysfunction in the amyloid precursor protein/presenilin 2 mouse model of Alzheimer's disease. J Neurosci 2007 Jan 24;27(4):824-31
2. Developmental regulation of the mouse IGF-I exon 1 promoter region by calcineurin activation of NFAT in skeletal muscle. Am J Physiol Cell Physiol 2007 Jan 17;
3. Effects of systemic and local administration of recombinant human IGF-I (rhIGF-I) on de novo bone formation in an aged mouse model. J Bone Miner Res 2006 Sep;21(9):1359-66
4. Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I. J Endocrinol 2006 May;189(2):279-87
5. Effects of GH and IGF-I on the in vitro maturation of mouse oocytes. Hormones (Athens) 2005 Jul-Sep;4(3):155-60
6. Recombinant human insulin-like growth factor-I treatment inhibits gluconeogenesis in a transgenic mouse model of type 2 diabetes mellitus. Endocrinology 2006 Jun;147(6):2619-3

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.